Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 116301..116824 Replicon plasmid P1
Accession NZ_OW970515
Organism Klebsiella pneumoniae isolate 147

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag LQ358_RS26465 Protein ID WP_015632464.1
Coordinates 116301..116606 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag LQ358_RS26470 Protein ID WP_015632465.1
Coordinates 116606..116824 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ358_RS26420 (AI2763V1_5016) 111331..111759 - 429 WP_015632456.1 plasmid partitioning/stability family protein -
LQ358_RS26425 (AI2763V1_5017) 111752..112732 - 981 WP_015632457.1 plasmid segregation protein ParM -
LQ358_RS26430 (AI2763V1_5018) 113093..113275 + 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
LQ358_RS26435 (AI2763V1_5019) 113301..113744 + 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
LQ358_RS26440 113950..114072 - 123 Protein_120 transcriptional regulator -
LQ358_RS26445 (AI2763V1_5021) 114086..114313 - 228 WP_016162072.1 hypothetical protein -
LQ358_RS26450 114545..115000 + 456 WP_015632461.1 helix-turn-helix domain-containing protein -
LQ358_RS26455 115252..115347 - 96 Protein_123 IS1 family transposase -
LQ358_RS26460 (AI2763V1_5024) 115812..116189 - 378 WP_016162069.1 hypothetical protein -
LQ358_RS26465 (AI2763V1_5025) 116301..116606 - 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
LQ358_RS26470 (AI2763V1_5026) 116606..116824 - 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LQ358_RS26475 (AI2763V1_5027) 117010..118035 + 1026 WP_015632466.1 IS110 family transposase -
LQ358_RS26480 (AI2763V1_REPA000000038) 118330..118815 + 486 Protein_128 IS5 family transposase -
LQ358_RS26485 (AI2763V1_5028) 118979..119410 + 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
LQ358_RS26490 (AI2763V1_5029) 119410..120681 + 1272 WP_016162068.1 Y-family DNA polymerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtF / ARR-3 - 1..121074 121074


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T296432 WP_015632464.1 NZ_OW970515:c116606-116301 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT296432 WP_015632465.1 NZ_OW970515:c116824-116606 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References