Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5214179..5214804 Replicon chromosome
Accession NZ_OW970514
Organism Klebsiella pneumoniae isolate 147

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LQ358_RS25405 Protein ID WP_002882817.1
Coordinates 5214179..5214562 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LQ358_RS25410 Protein ID WP_004150355.1
Coordinates 5214562..5214804 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ358_RS25390 (5211545) 5211545..5212447 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LQ358_RS25395 (5212444) 5212444..5213079 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LQ358_RS25400 (5213076) 5213076..5214005 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LQ358_RS25405 (5214179) 5214179..5214562 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ358_RS25410 (5214562) 5214562..5214804 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LQ358_RS25415 (5215009) 5215009..5215926 + 918 WP_021466676.1 alpha/beta hydrolase -
LQ358_RS25420 (5215940) 5215940..5216881 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LQ358_RS25425 (5216926) 5216926..5217363 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LQ358_RS25430 (5217360) 5217360..5218220 - 861 WP_004146232.1 virulence factor BrkB family protein -
LQ358_RS25435 (5218214) 5218214..5218813 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T296431 WP_002882817.1 NZ_OW970514:c5214562-5214179 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT296431 WP_004150355.1 NZ_OW970514:c5214804-5214562 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References