Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4709852..4710662 Replicon chromosome
Accession NZ_OW970444
Organism Klebsiella pneumoniae isolate 101

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LQV08_RS23145 Protein ID WP_004178461.1
Coordinates 4709852..4710385 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LQV08_RS23150 Protein ID WP_002887278.1
Coordinates 4710396..4710662 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV08_RS23140 (4708683) 4708683..4709804 + 1122 WP_004214138.1 cupin domain-containing protein -
LQV08_RS23145 (4709852) 4709852..4710385 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LQV08_RS23150 (4710396) 4710396..4710662 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LQV08_RS23155 (4710765) 4710765..4712198 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
LQV08_RS23160 (4712188) 4712188..4712871 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LQV08_RS23165 (4713043) 4713043..4714428 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
LQV08_RS23170 (4714446) 4714446..4714790 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T296346 WP_004178461.1 NZ_OW970444:c4710385-4709852 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT296346 WP_002887278.1 NZ_OW970444:c4710662-4710396 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References