Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4733211..4734021 Replicon chromosome
Accession NZ_OW970374
Organism Klebsiella pneumoniae isolate 101

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LQV21_RS23250 Protein ID WP_004178461.1
Coordinates 4733211..4733744 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LQV21_RS23255 Protein ID WP_002887278.1
Coordinates 4733755..4734021 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV21_RS23245 (4732042) 4732042..4733163 + 1122 WP_004214138.1 cupin domain-containing protein -
LQV21_RS23250 (4733211) 4733211..4733744 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LQV21_RS23255 (4733755) 4733755..4734021 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LQV21_RS23260 (4734124) 4734124..4735557 - 1434 WP_032418099.1 Cu(+)/Ag(+) sensor histidine kinase -
LQV21_RS23265 (4735547) 4735547..4736230 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LQV21_RS23270 (4736402) 4736402..4737787 + 1386 WP_032418101.1 efflux transporter outer membrane subunit -
LQV21_RS23275 (4737805) 4737805..4738149 + 345 WP_032418103.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T296282 WP_004178461.1 NZ_OW970374:c4733744-4733211 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT296282 WP_002887278.1 NZ_OW970374:c4734021-4733755 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References