Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5240561..5241186 Replicon chromosome
Accession NZ_OW970357
Organism Klebsiella pneumoniae isolate 147

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LQV29_RS25525 Protein ID WP_002882817.1
Coordinates 5240561..5240944 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LQV29_RS25530 Protein ID WP_004150355.1
Coordinates 5240944..5241186 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQV29_RS25510 (5237927) 5237927..5238829 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LQV29_RS25515 (5238826) 5238826..5239461 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LQV29_RS25520 (5239458) 5239458..5240387 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LQV29_RS25525 (5240561) 5240561..5240944 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LQV29_RS25530 (5240944) 5240944..5241186 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LQV29_RS25535 (5241391) 5241391..5242308 + 918 WP_021466676.1 alpha/beta hydrolase -
LQV29_RS25540 (5242322) 5242322..5243263 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LQV29_RS25545 (5243308) 5243308..5243745 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LQV29_RS25550 (5243742) 5243742..5244602 - 861 WP_004146232.1 virulence factor BrkB family protein -
LQV29_RS25555 (5244596) 5244596..5245195 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T296253 WP_002882817.1 NZ_OW970357:c5240944-5240561 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT296253 WP_004150355.1 NZ_OW970357:c5241186-5240944 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References