Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1770117..1770707 Replicon chromosome
Accession NZ_OW969912
Organism Klebsiella variicola isolate 0

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag LQ196_RS08515 Protein ID WP_008804165.1
Coordinates 1770375..1770707 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag LQ196_RS08510 Protein ID WP_012541132.1
Coordinates 1770117..1770374 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ196_RS08490 (AN2340V1_1625) 1765726..1766301 + 576 WP_032730762.1 hypothetical protein -
LQ196_RS08495 (AN2340V1_1626) 1766479..1767315 + 837 WP_008804155.1 alpha/beta hydrolase -
LQ196_RS08500 (AN2340V1_1627) 1767521..1768492 + 972 WP_016161433.1 sensor domain-containing diguanylate cyclase -
LQ196_RS08505 (AN2340V1_1628) 1768489..1769589 - 1101 WP_117110384.1 AarF/UbiB family protein -
LQ196_RS08510 (AN2340V1_1629) 1770117..1770374 + 258 WP_012541132.1 antitoxin Antitoxin
LQ196_RS08515 (AN2340V1_1630) 1770375..1770707 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ196_RS08525 (AN2340V1_1631) 1771030..1772466 + 1437 WP_042948427.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LQ196_RS08535 (AN2340V1_1632) 1772839..1774293 - 1455 WP_008804170.1 AMP nucleosidase -
LQ196_RS08540 (AN2340V1_1633) 1774424..1774669 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T296218 WP_008804165.1 NZ_OW969912:1770375-1770707 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT296218 WP_012541132.1 NZ_OW969912:1770117-1770374 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References