Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60301..60944 Replicon plasmid P1
Accession NZ_OW968432
Organism Klebsiella quasipneumoniae isolate 0

Toxin (Protein)


Gene name vagD Uniprot ID A0A6H2GRP8
Locus tag LQ152_RS25065 Protein ID WP_023292131.1
Coordinates 60528..60944 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag LQ152_RS25060 Protein ID WP_021312476.1
Coordinates 60301..60531 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ152_RS25050 (AI3010V1_4778) 57007..57090 - 84 Protein_60 HEPN family nuclease -
LQ152_RS25055 (AI3010V1_4779) 57458..59668 + 2211 WP_023292130.1 AAA family ATPase -
LQ152_RS25060 (AI3010V1_4780) 60301..60531 + 231 WP_021312476.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LQ152_RS25065 (AI3010V1_4781) 60528..60944 + 417 WP_023292131.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ152_RS25070 (AI3010V1_4782) 61042..61368 + 327 WP_023292132.1 hypothetical protein -
LQ152_RS25075 (AI3010V1_REPA000000008) 61437..61598 - 162 Protein_65 IS1 family transposase -
LQ152_RS25080 (AI3010V1_REPA000000007) 61656..62558 - 903 Protein_66 IS5 family transposase -
LQ152_RS25085 (AI3010V1_4784) 62669..62941 - 273 WP_023292134.1 YkgJ family cysteine cluster protein -
LQ152_RS25090 (AI3010V1_4785) 62938..64509 - 1572 WP_023292135.1 hypothetical protein -
LQ152_RS25095 (AI3010V1_4786) 64556..65653 - 1098 WP_023292136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..131788 131788
- inside IScluster/Tn - - 55798..66808 11010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14975.40 Da        Isoelectric Point: 8.5464

>T296139 WP_023292131.1 NZ_OW968432:60528-60944 [Klebsiella quasipneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT296139 WP_021312476.1 NZ_OW968432:60301-60531 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GRP8

Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References