Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 39908..40711 Replicon plasmid P2
Accession NZ_OW968419
Organism Enterobacter cloacae isolate 764

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag LQ125_RS24300 Protein ID WP_022652313.1
Coordinates 40181..40711 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag LQ125_RS24295 Protein ID WP_022652312.1
Coordinates 39908..40177 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ125_RS24275 (AI3013V2_4710) 35665..36633 + 969 WP_000654812.1 IS5-like element IS903B family transposase -
LQ125_RS24280 36754..36822 + 69 Protein_44 serine/threonine protein kinase -
LQ125_RS24285 (AI3013V2_4711) 36997..37737 + 741 WP_022652310.1 site-specific integrase -
LQ125_RS24290 (AI3013V2_4712) 38063..39052 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
LQ125_RS24295 (AI3013V2_4713) 39908..40177 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
LQ125_RS24300 (AI3013V2_4714) 40181..40711 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
LQ125_RS24305 (AI3013V2_REPA000000083) 40817..41514 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
LQ125_RS24310 41537..41935 - 399 Protein_50 cytoplasmic protein -
LQ125_RS24315 41934..42482 + 549 Protein_51 hypothetical protein -
LQ125_RS24320 42678..42932 - 255 Protein_52 hypothetical protein -
LQ125_RS24325 42850..43329 + 480 WP_229694441.1 3'-5' exonuclease -
LQ125_RS24330 (AI3013V2_4721) 43382..43777 + 396 WP_040219261.1 hypothetical protein -
LQ125_RS24335 (AI3013V2_4722) 43774..44385 + 612 WP_015493086.1 DUF2913 family protein -
LQ125_RS24340 (AI3013V2_4723) 44382..45332 - 951 WP_040219234.1 Rpn family recombination-promoting nuclease/putative transposase -
LQ125_RS24345 45479..45679 - 201 WP_040219232.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / aadA2 mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..196573 196573
- inside IScluster/Tn - - 35665..41514 5849


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T296126 WP_022652313.1 NZ_OW968419:40181-40711 [Enterobacter cloacae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT296126 WP_022652312.1 NZ_OW968419:39908-40177 [Enterobacter cloacae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References