Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 223245..223771 Replicon plasmid P1
Accession NZ_OW968418
Organism Enterobacter cloacae isolate 764

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LQ125_RS23730 Protein ID WP_000323025.1
Coordinates 223245..223532 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LQ125_RS23735 Protein ID WP_000534858.1
Coordinates 223532..223771 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ125_RS23700 218815..218991 - 177 WP_001371930.1 hypothetical protein -
LQ125_RS23705 (AI3013V2_4596) 219502..220446 + 945 WP_000778029.1 DUF5417 domain-containing protein -
LQ125_RS23710 (AI3013V2_4597) 220542..221144 + 603 WP_001371889.1 hypothetical protein -
LQ125_RS23715 (AI3013V2_4598) 221204..221554 + 351 WP_000743059.1 hypothetical protein -
LQ125_RS23720 (AI3013V2_4600) 222086..222406 + 321 WP_000332796.1 hypothetical protein -
LQ125_RS23725 (AI3013V2_4601) 223019..223144 - 126 WP_229020258.1 DUF5431 family protein -
LQ125_RS23730 (AI3013V2_4602) 223245..223532 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LQ125_RS23735 (AI3013V2_4603) 223532..223771 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LQ125_RS23740 223796..223900 + 105 Protein_231 protein YdfV -
LQ125_RS23745 (AI3013V2_4604) 224034..224957 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
LQ125_RS23750 (AI3013V2_4605) 225157..225729 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
LQ125_RS23755 (AI3013V2_4606) 226205..227443 - 1239 WP_000219087.1 IS110-like element ISEsa2 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / blaVIM-1 / dfrB1 / ant(3'')-Ia / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / blaCTX-M-9 / mcr-9 - 1..281280 281280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T296125 WP_000323025.1 NZ_OW968418:c223532-223245 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT296125 WP_000534858.1 NZ_OW968418:c223771-223532 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References