Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 93052..94675 Replicon plasmid P1
Accession NZ_OW968418
Organism Enterobacter cloacae isolate 764

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LQ125_RS23025 Protein ID WP_000088044.1
Coordinates 93350..94675 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LQ125_RS23020 Protein ID WP_012477377.1
Coordinates 93052..93345 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ125_RS23000 (AI3013V2_4452) 88851..89243 + 393 WP_000341066.1 hypothetical protein -
LQ125_RS23005 (AI3013V2_4453) 89902..90558 + 657 WP_000462754.1 trypsin-like serine protease -
LQ125_RS23010 (AI3013V2_4454) 90761..91258 + 498 WP_000340139.1 membrane protein -
LQ125_RS23015 (AI3013V2_4455) 91263..92651 + 1389 WP_012695445.1 hypothetical protein -
LQ125_RS23020 (AI3013V2_4456) 93052..93345 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LQ125_RS23025 (AI3013V2_4457) 93350..94675 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LQ125_RS23030 (AI3013V2_4458) 94736..94942 + 207 WP_000134171.1 hypothetical protein -
LQ125_RS23035 (AI3013V2_4459) 95043..95453 + 411 WP_000985909.1 hypothetical protein -
LQ125_RS23040 (AI3013V2_4460) 95466..95990 + 525 WP_001805097.1 hypothetical protein -
LQ125_RS23045 (AI3013V2_4461) 96172..97176 - 1005 WP_022542389.1 IS110-like element IS4321 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / qnrA1 / tet(A) / blaVIM-1 / dfrB1 / ant(3'')-Ia / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / blaCTX-M-9 / mcr-9 - 1..281280 281280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T296124 WP_000088044.1 NZ_OW968418:93350-94675 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT296124 WP_012477377.1 NZ_OW968418:93052-93345 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References