296028

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2076959..2077535 Replicon chromosome
Accession NZ_OW968277
Organism Escherichia coli isolate 131

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag LQ166_RS10385 Protein ID WP_001295743.1
Coordinates 2077248..2077535 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag LQ166_RS10380 Protein ID WP_000063148.1
Coordinates 2076959..2077261 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ166_RS10365 (2073555) 2073555..2075705 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LQ166_RS10370 (2075755) 2075755..2075958 + 204 WP_000467859.1 YbdD/YjiX family protein -
LQ166_RS10375 (2075969) 2075969..2076925 + 957 WP_001295745.1 GTPase -
LQ166_RS10380 (2076959) 2076959..2077261 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
LQ166_RS10385 (2077248) 2077248..2077535 - 288 WP_001295743.1 BrnT family toxin Toxin
LQ166_RS10390 (2077732) 2077732..2078898 + 1167 WP_000800831.1 restriction endonuclease -
LQ166_RS10395 (2078962) 2078962..2080581 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
LQ166_RS10400 (2080571) 2080571..2081875 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T296028 WP_001295743.1 NZ_OW968277:c2077535-2077248 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT296028 WP_000063148.1 NZ_OW968277:c2077261-2076959 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References