Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4143466..4144042 Replicon chromosome
Accession NZ_OW968124
Organism Escherichia coli isolate 10

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LQ159_RS20150 Protein ID WP_001297643.1
Coordinates 4143755..4144042 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LQ159_RS20145 Protein ID WP_000063156.1
Coordinates 4143466..4143768 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ159_RS20130 (4140062) 4140062..4142212 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LQ159_RS20135 (4142262) 4142262..4142465 + 204 WP_000467859.1 YbdD/YjiX family protein -
LQ159_RS20140 (4142476) 4142476..4143432 + 957 WP_001297640.1 GTPase -
LQ159_RS20145 (4143466) 4143466..4143768 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LQ159_RS20150 (4143755) 4143755..4144042 - 288 WP_001297643.1 BrnT family toxin Toxin
LQ159_RS20155 (4144241) 4144241..4144938 - 698 Protein_3962 IS1 family transposase -
LQ159_RS20160 (4144995) 4144995..4145090 + 96 Protein_3963 dienelactone hydrolase family protein -
LQ159_RS20165 (4145236) 4145236..4146468 + 1233 WP_063104094.1 multidrug efflux MFS transporter MdtM -
LQ159_RS20170 (4146509) 4146509..4147789 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 4074342..4144744 70402


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T296002 WP_001297643.1 NZ_OW968124:c4144042-4143755 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT296002 WP_000063156.1 NZ_OW968124:c4143768-4143466 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References