295958

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4189569..4190406 Replicon chromosome
Accession NZ_OW968011
Organism Escherichia coli isolate 58

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag LQ149_RS19990 Protein ID WP_000227784.1
Coordinates 4189569..4190111 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag LQ149_RS19995 Protein ID WP_001297137.1
Coordinates 4190095..4190406 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ149_RS19970 (4185108) 4185108..4186019 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
LQ149_RS19975 (4186187) 4186187..4186678 + 492 WP_001138904.1 nucleotide binding protein YajQ -
LQ149_RS19980 (4186806) 4186806..4188170 - 1365 WP_001000978.1 MFS transporter -
LQ149_RS19985 (4188578) 4188578..4189513 + 936 WP_001297127.1 tetratricopeptide repeat protein -
LQ149_RS19990 (4189569) 4189569..4190111 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
LQ149_RS19995 (4190095) 4190095..4190406 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
LQ149_RS20000 (4190591) 4190591..4191481 - 891 WP_000971336.1 heme o synthase -
LQ149_RS20005 (4191493) 4191493..4191822 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LQ149_RS20010 (4191822) 4191822..4192436 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LQ149_RS20015 (4192426) 4192426..4194417 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LQ149_RS20020 (4194439) 4194439..4195386 - 948 WP_021548571.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T295958 WP_000227784.1 NZ_OW968011:c4190111-4189569 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT295958 WP_001297137.1 NZ_OW968011:c4190406-4190095 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References