Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3431230..3431727 Replicon chromosome
Accession NZ_OW968011
Organism Escherichia coli isolate 58

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LQ149_RS16415 Protein ID WP_001173343.1
Coordinates 3431554..3431727 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LQ149_RS16410 Protein ID WP_000398619.1
Coordinates 3431230..3431526 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ149_RS16390 (3427961) 3427961..3428680 - 720 WP_000611288.1 two-component system response regulator DcuR -
LQ149_RS16395 (3428677) 3428677..3430308 - 1632 WP_001216461.1 sensor histidine kinase -
LQ149_RS16400 (3430489) 3430489..3430719 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
LQ149_RS16405 (3430731) 3430731..3431003 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
LQ149_RS16410 (3431230) 3431230..3431526 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LQ149_RS16415 (3431554) 3431554..3431727 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LQ149_RS16420 (3431846) 3431846..3433363 - 1518 WP_001295074.1 lysine--tRNA ligase -
LQ149_RS16425 (3433600) 3433600..3435057 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T295955 WP_001173343.1 NZ_OW968011:3431554-3431727 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT295955 WP_000398619.1 NZ_OW968011:3431230-3431526 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References