Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4135598..4136174 Replicon chromosome
Accession NZ_OW967975
Organism Escherichia coli isolate 10

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LQ150_RS20085 Protein ID WP_001297643.1
Coordinates 4135887..4136174 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LQ150_RS20080 Protein ID WP_000063156.1
Coordinates 4135598..4135900 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ150_RS20065 (4132194) 4132194..4134344 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LQ150_RS20070 (4134394) 4134394..4134597 + 204 WP_000467859.1 YbdD/YjiX family protein -
LQ150_RS20075 (4134608) 4134608..4135564 + 957 WP_001297640.1 GTPase -
LQ150_RS20080 (4135598) 4135598..4135900 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LQ150_RS20085 (4135887) 4135887..4136174 - 288 WP_001297643.1 BrnT family toxin Toxin
LQ150_RS20095 (4137127) 4137127..4137222 + 96 Protein_3950 dienelactone hydrolase family protein -
LQ150_RS20100 (4137368) 4137368..4138600 + 1233 WP_063104094.1 multidrug efflux MFS transporter MdtM -
LQ150_RS20105 (4138641) 4138641..4139921 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4136373..4136666 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T295917 WP_001297643.1 NZ_OW967975:c4136174-4135887 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT295917 WP_000063156.1 NZ_OW967975:c4135900-4135598 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References