Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 92901..93544 Replicon plasmid P1
Accession NZ_OW967967
Organism Escherichia coli isolate 648

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag LQ127_RS25470 Protein ID WP_001044768.1
Coordinates 93128..93544 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag LQ127_RS25465 Protein ID WP_001261287.1
Coordinates 92901..93131 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ127_RS25455 (AI2858V1_4933) 88079..89212 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
LQ127_RS25460 (AI2858V1_4934) 89475..92594 - 3120 WP_023909028.1 hypothetical protein -
LQ127_RS25465 (AI2858V1_4935) 92901..93131 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LQ127_RS25470 (AI2858V1_4936) 93128..93544 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ127_RS25475 (AI2858V1_4937) 93706..95844 - 2139 WP_000350635.1 AAA family ATPase -
LQ127_RS25480 (AI2858V1_4938) 96309..97304 + 996 WP_000246636.1 hypothetical protein -
LQ127_RS25485 (AI2858V1_4939) 97308..98240 + 933 WP_000991832.1 S-4TM family putative pore-forming effector -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / mph(A) / erm(B) - 1..107837 107837
- flank IS/Tn - - 98377..98880 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T295900 WP_001044768.1 NZ_OW967967:93128-93544 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT295900 WP_001261287.1 NZ_OW967967:92901-93131 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References