Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4537654..4538151 Replicon chromosome
Accession NZ_OW967966
Organism Escherichia coli isolate 648

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LQ127_RS22195 Protein ID WP_001173343.1
Coordinates 4537654..4537827 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LQ127_RS22200 Protein ID WP_000398619.1
Coordinates 4537855..4538151 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ127_RS22185 (4534324) 4534324..4535781 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
LQ127_RS22190 (4536018) 4536018..4537535 + 1518 WP_022646456.1 lysine--tRNA ligase -
LQ127_RS22195 (4537654) 4537654..4537827 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LQ127_RS22200 (4537855) 4537855..4538151 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LQ127_RS22205 (4538380) 4538380..4538652 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LQ127_RS22210 (4538664) 4538664..4538894 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
LQ127_RS22215 (4539075) 4539075..4540706 + 1632 WP_022646455.1 sensor histidine kinase -
LQ127_RS22220 (4540703) 4540703..4541422 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4528800..4538151 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T295896 WP_001173343.1 NZ_OW967966:c4537827-4537654 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT295896 WP_000398619.1 NZ_OW967966:c4538151-4537855 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References