Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4333865..4334441 Replicon chromosome
Accession NZ_OW967966
Organism Escherichia coli isolate 648

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LQ127_RS21175 Protein ID WP_001297643.1
Coordinates 4334154..4334441 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag LQ127_RS21170 Protein ID WP_000063156.1
Coordinates 4333865..4334167 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ127_RS21155 (4330461) 4330461..4332611 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
LQ127_RS21160 (4332661) 4332661..4332864 + 204 WP_000467859.1 YbdD/YjiX family protein -
LQ127_RS21165 (4332875) 4332875..4333831 + 957 WP_001297640.1 GTPase -
LQ127_RS21170 (4333865) 4333865..4334167 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
LQ127_RS21175 (4334154) 4334154..4334441 - 288 WP_001297643.1 BrnT family toxin Toxin
LQ127_RS21180 (4334640) 4334640..4335337 - 698 Protein_4163 IS1 family transposase -
LQ127_RS21185 (4335394) 4335394..4335489 + 96 Protein_4164 dienelactone hydrolase family protein -
LQ127_RS21190 (4335635) 4335635..4336867 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
LQ127_RS21195 (4336908) 4336908..4338188 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T295894 WP_001297643.1 NZ_OW967966:c4334441-4334154 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT295894 WP_000063156.1 NZ_OW967966:c4334167-4333865 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References