Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3053351..3054135 Replicon chromosome
Accession NZ_OW967966
Organism Escherichia coli isolate 648

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LQ127_RS14935 Protein ID WP_000613626.1
Coordinates 3053641..3054135 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag LQ127_RS14930 Protein ID WP_024946541.1
Coordinates 3053351..3053644 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ127_RS14920 (3048512) 3048512..3049471 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
LQ127_RS14925 (3050044) 3050044..3053217 + 3174 WP_022645526.1 ribonuclease E -
LQ127_RS14930 (3053351) 3053351..3053644 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
LQ127_RS14935 (3053641) 3053641..3054135 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LQ127_RS14940 (3054230) 3054230..3055183 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
LQ127_RS14945 (3055195) 3055195..3056838 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
LQ127_RS14950 (3056904) 3056904..3057845 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LQ127_RS14955 (3057845) 3057845..3058942 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T295889 WP_000613626.1 NZ_OW967966:3053641-3054135 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT295889 WP_024946541.1 NZ_OW967966:3053351-3053644 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References