Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 67238..67974 Replicon 2 plasmid P1
Accession NZ_OW967519
Organism Klebsiella oxytoca isolate

Toxin (Protein)


Gene name tacT Uniprot ID J5UJ49
Locus tag LQ214_RS28950 Protein ID WP_009654334.1
Coordinates 67492..67974 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LQ214_RS28945 Protein ID WP_003026799.1
Coordinates 67238..67504 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ214_RS28925 (AI2744V1_5651) 63238..64206 - 969 WP_077266818.1 IS5 family transposase -
LQ214_RS28930 (AI2744V1_5652) 64186..65217 + 1032 WP_064352057.1 IS110 family transposase -
LQ214_RS28935 (AI2744V1_5653) 65536..66504 - 969 WP_077267151.1 IS5 family transposase -
LQ214_RS28940 (AI2744V1_5654) 66565..66999 + 435 WP_042928448.1 cell envelope integrity protein TolA -
LQ214_RS28945 (AI2744V1_5655) 67238..67504 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LQ214_RS28950 (AI2744V1_5656) 67492..67974 + 483 WP_009654334.1 GNAT family N-acetyltransferase Toxin
LQ214_RS28955 (AI2744V1_5657) 68181..69527 + 1347 WP_077266289.1 ISNCY family transposase -
LQ214_RS28960 (AI2744V1_5658) 69576..69974 + 399 WP_032422684.1 helix-turn-helix domain-containing protein -
LQ214_RS28965 (AI2744V1_REPA000000036) 70338..70472 - 135 Protein_67 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..195319 195319
- inside IScluster/Tn - - 63238..79449 16211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17339.06 Da        Isoelectric Point: 8.7400

>T295822 WP_009654334.1 NZ_OW967519:67492-67974 [Klebsiella oxytoca]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHVKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT295822 WP_003026799.1 NZ_OW967519:67238-67504 [Klebsiella oxytoca]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4QCA1

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References