Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 107344..108967 Replicon plasmid P1
Accession NZ_OW967389
Organism Enterobacter cloacae isolate 114

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag LQ218_RS23540 Protein ID WP_000088044.1
Coordinates 107642..108967 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag LQ218_RS23535 Protein ID WP_012477377.1
Coordinates 107344..107637 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ218_RS23515 (AI2624V1_4564) 103143..103535 + 393 WP_000341066.1 hypothetical protein -
LQ218_RS23520 (AI2624V1_4565) 104194..104850 + 657 WP_000462754.1 trypsin-like serine protease -
LQ218_RS23525 (AI2624V1_4566) 105053..105550 + 498 WP_000340139.1 membrane protein -
LQ218_RS23530 (AI2624V1_4567) 105555..106943 + 1389 WP_000083579.1 hypothetical protein -
LQ218_RS23535 (AI2624V1_4568) 107344..107637 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
LQ218_RS23540 (AI2624V1_4569) 107642..108967 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
LQ218_RS23545 (AI2624V1_4570) 109028..109234 + 207 WP_000134171.1 hypothetical protein -
LQ218_RS23550 (AI2624V1_4571) 109451..110431 - 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -
LQ218_RS23555 (AI2624V1_4572) 110477..111100 - 624 WP_000490639.1 hypothetical protein -
LQ218_RS23560 (AI2624V1_4573) 111158..111538 - 381 WP_001371925.1 hypothetical protein -
LQ218_RS23565 112293..112463 + 171 Protein_114 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..213001 213001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T295812 WP_000088044.1 NZ_OW967389:107642-108967 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT295812 WP_012477377.1 NZ_OW967389:107344-107637 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References