Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA(toxin)
Location 3117190..3118973 Replicon chromosome
Accession NZ_OW967388
Organism Enterobacter cloacae isolate 114

Toxin (Protein)


Gene name hipA Uniprot ID A0A8B5ZXW9
Locus tag LQ218_RS15095 Protein ID WP_022651516.1
Coordinates 3117190..3118539 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A837FFV9
Locus tag LQ218_RS15100 Protein ID WP_003859317.1
Coordinates 3118536..3118973 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ218_RS15070 (AI2624V1_2935) 3113013..3113948 - 936 WP_003859306.1 tRNA dihydrouridine(16) synthase DusC -
LQ218_RS15075 (AI2624V1_2936) 3114117..3114512 + 396 WP_003859308.1 CidA/LrgA family protein -
LQ218_RS15080 (AI2624V1_2937) 3114509..3115204 + 696 WP_003859310.1 CidB/LrgB family autolysis modulator -
LQ218_RS15085 (AI2624V1_2938) 3115333..3116217 + 885 WP_022651515.1 cytidine deaminase -
LQ218_RS15090 (AI2624V1_2939) 3116345..3117055 + 711 WP_250249391.1 outer membrane permeability protein SanA -
LQ218_RS15095 (AI2624V1_2940) 3117190..3118539 + 1350 WP_022651516.1 HipA domain-containing protein Toxin
LQ218_RS15100 (AI2624V1_2941) 3118536..3118973 + 438 WP_003859317.1 helix-turn-helix transcriptional regulator Antitoxin
LQ218_RS15105 (AI2624V1_2942) 3119012..3120022 - 1011 WP_003859318.1 galactose/methyl galactoside ABC transporter permease MglC -
LQ218_RS15110 (AI2624V1_2943) 3120038..3121558 - 1521 WP_003859320.1 galactose/methyl galactoside ABC transporter ATP-binding protein MglA -
LQ218_RS15115 (AI2624V1_2944) 3121638..3122636 - 999 WP_003859322.1 galactose/glucose ABC transporter substrate-binding protein MglB -
LQ218_RS15120 (AI2624V1_2945) 3122932..3123954 - 1023 WP_003859324.1 HTH-type transcriptional regulator GalS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(166-406)

Antitoxin

Cro/C1-type helix-turn-helix domain

(75-128)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 51151.85 Da        Isoelectric Point: 4.7049

>T295810 WP_022651516.1 NZ_OW967388:3117190-3118539 [Enterobacter cloacae]
METLTVQAYLNETWTDIALIKYPGSEKKDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Download         Length: 1350 bp

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16185.64 Da        Isoelectric Point: 10.5154

>AT295810 WP_003859317.1 NZ_OW967388:3118536-3118973 [Enterobacter cloacae]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Download         Length: 438 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5ZXW9

Antitoxin

Source ID Structure
AlphaFold DB A0A837FFV9

References