295782

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4027453..4028132 Replicon chromosome
Accession NZ_OW967263
Organism Citrobacter freundii isolate 22

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag LQ176_RS19710 Protein ID WP_003031349.1
Coordinates 4027791..4028132 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag LQ176_RS19705 Protein ID WP_003031347.1
Coordinates 4027453..4027770 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ176_RS19680 (4023546) 4023546..4025543 - 1998 WP_016245729.1 choline BCCT transporter BetT -
LQ176_RS19685 (4026091) 4026091..4026238 + 148 Protein_3866 hypothetical protein -
LQ176_RS19690 (4026252) 4026252..4026713 + 462 WP_003031344.1 antirestriction protein -
LQ176_RS19695 (4026729) 4026729..4027205 + 477 WP_003031345.1 RadC family protein -
LQ176_RS19700 (4027214) 4027214..4027435 + 222 WP_003031346.1 DUF987 domain-containing protein -
LQ176_RS19705 (4027453) 4027453..4027770 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LQ176_RS19710 (4027791) 4027791..4028132 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
LQ176_RS19715 (4028722) 4028722..4029324 - 603 WP_230133428.1 tail fiber assembly protein -
LQ176_RS19720 (4029326) 4029326..4030627 - 1302 WP_230133429.1 hypothetical protein -
LQ176_RS19725 (4030627) 4030627..4031307 - 681 WP_044702567.1 DUF2612 domain-containing protein -
LQ176_RS19730 (4031304) 4031304..4032503 - 1200 WP_044702568.1 baseplate J/gp47 family protein -
LQ176_RS19735 (4032504) 4032504..4032857 - 354 WP_016239889.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4026252..4077711 51459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T295782 WP_003031349.1 NZ_OW967263:4027791-4028132 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT295782 WP_003031347.1 NZ_OW967263:4027453-4027770 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References