Detailed information of TA system TA029575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2180609..2181138
• Replicon: chromosome
• Accession: NC_021554
• Organism: Staphylococcus aureus CA-347

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CA347_RS11095
• Protein ID: WP_000621175.1
• Coordinates: 2180609..2180971 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CA347_RS11100
• Protein ID: WP_000948331.1
• Coordinates: 2180968..2181138 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA347_RS11070	2177587..2178357	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
CA347_RS11075	2178332..2178811	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
CA347_RS11080	2178813..2179139	-	327	WP_001052491.1	anti-sigma factor antagonist	-
CA347_RS11085	2179258..2180259	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CA347_RS11095	2180609..2180971	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CA347_RS11100	2180968..2181138	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CA347_RS11105	2181223..2182371	-	1149	WP_001281140.1	alanine racemase	-
CA347_RS11110	2182437..2182796	-	360	WP_000581199.1	holo-ACP synthase	-
CA347_RS11115	2182800..2183291	-	492	WP_001205907.1	PH domain-containing protein	-
CA347_RS11120	2183278..2184861	-	1584	WP_001294637.1	PH domain-containing protein	-
CA347_RS11125	2184854..2185333	-	480	WP_001287077.1	hypothetical protein	-
CA347_RS11130	2185541..2186101	-	561	WP_001092406.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T29575 WP_000621175.1 NC_021554:c2180971-2180609 [Staphylococcus aureus CA-347]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T29575 NC_021554:c2180971-2180609 [Staphylococcus aureus CA-347]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT29575 WP_000948331.1 NC_021554:c2181138-2180968 [Staphylococcus aureus CA-347]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT29575 NC_021554:c2181138-2180968 [Staphylococcus aureus CA-347]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1