Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2672748..2673245 Replicon chromosome
Accession NZ_OW967003
Organism Escherichia coli isolate 624

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LQ179_RS12825 Protein ID WP_001173343.1
Coordinates 2672748..2672921 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LQ179_RS12830 Protein ID WP_000398619.1
Coordinates 2672949..2673245 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ179_RS12815 (2669418) 2669418..2670875 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
LQ179_RS12820 (2671112) 2671112..2672629 + 1518 WP_022646456.1 lysine--tRNA ligase -
LQ179_RS12825 (2672748) 2672748..2672921 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LQ179_RS12830 (2672949) 2672949..2673245 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LQ179_RS12835 (2673474) 2673474..2673746 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LQ179_RS12840 (2673758) 2673758..2673988 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
LQ179_RS12845 (2674169) 2674169..2675800 + 1632 WP_022646455.1 sensor histidine kinase -
LQ179_RS12850 (2675797) 2675797..2676516 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2663894..2673245 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T295744 WP_001173343.1 NZ_OW967003:c2672921-2672748 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT295744 WP_000398619.1 NZ_OW967003:c2673245-2672949 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References