Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 79545..80348 Replicon plasmid P2
Accession NZ_OW849529
Organism Citrobacter freundii isolate 22

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag LQ181_RS26845 Protein ID WP_022652313.1
Coordinates 79818..80348 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag LQ181_RS26840 Protein ID WP_022652312.1
Coordinates 79545..79814 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ181_RS26810 (AI2661V1_5223) 74610..74840 - 231 WP_023307222.1 hypothetical protein -
LQ181_RS26815 (AI2661V1_5224) 74854..75057 - 204 WP_004213596.1 HHA domain-containing protein -
LQ181_RS26820 (AI2661V1_5225) 75302..76270 + 969 WP_023307223.1 IS5-like element IS903B family transposase -
LQ181_RS26825 76391..76459 + 69 Protein_77 serine/threonine protein kinase -
LQ181_RS26830 (AI2661V1_5226) 76634..77374 + 741 WP_022652310.1 site-specific integrase -
LQ181_RS26835 (AI2661V1_5227) 77700..78689 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
LQ181_RS26840 (AI2661V1_5228) 79545..79814 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
LQ181_RS26845 (AI2661V1_5229) 79818..80348 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
LQ181_RS26850 (AI2661V1_5230) 80516..81373 + 858 WP_022652314.1 IS5 family transposase -
LQ181_RS26855 (AI2661V1_REPA000000146) 81514..82407 - 894 Protein_83 IS3 family transposase -
LQ181_RS26860 (AI2661V1_5232) 82475..83626 + 1152 WP_022652315.1 IS30-like element IS30 family transposase -
LQ181_RS26865 (AI2661V1_5234) 84115..84462 + 348 WP_000609174.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..149443 149443
- inside IScluster/Tn - - 64491..87917 23426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T295730 WP_022652313.1 NZ_OW849529:79818-80348 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT295730 WP_022652312.1 NZ_OW849529:79545-79814 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References