Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 73429..74165 Replicon plasmid P2
Accession NZ_OW849529
Organism Citrobacter freundii isolate 22

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LQ181_RS26800 Protein ID WP_003026803.1
Coordinates 73429..73911 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LQ181_RS26805 Protein ID WP_003026799.1
Coordinates 73899..74165 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ181_RS26780 (AI2661V1_5217) 68727..69530 - 804 WP_059509655.1 tetratricopeptide repeat protein -
LQ181_RS26785 (AI2661V1_5218) 69591..70937 - 1347 WP_080400673.1 ISNCY family transposase -
LQ181_RS26790 (AI2661V1_5219) 71164..71796 + 633 WP_001567369.1 hypothetical protein -
LQ181_RS26795 (AI2661V1_5220) 71825..73228 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LQ181_RS26800 (AI2661V1_5221) 73429..73911 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LQ181_RS26805 (AI2661V1_5222) 73899..74165 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LQ181_RS26810 (AI2661V1_5223) 74610..74840 - 231 WP_023307222.1 hypothetical protein -
LQ181_RS26815 (AI2661V1_5224) 74854..75057 - 204 WP_004213596.1 HHA domain-containing protein -
LQ181_RS26820 (AI2661V1_5225) 75302..76270 + 969 WP_023307223.1 IS5-like element IS903B family transposase -
LQ181_RS26825 76391..76459 + 69 Protein_77 serine/threonine protein kinase -
LQ181_RS26830 (AI2661V1_5226) 76634..77374 + 741 WP_022652310.1 site-specific integrase -
LQ181_RS26835 (AI2661V1_5227) 77700..78689 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) - 1..149443 149443
- inside IScluster/Tn - - 64491..87917 23426


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T295729 WP_003026803.1 NZ_OW849529:c73911-73429 [Citrobacter freundii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT295729 WP_003026799.1 NZ_OW849529:c74165-73899 [Citrobacter freundii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References