Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1335666..1336291 Replicon chromosome
Accession NZ_OW849377
Organism Escherichia coli isolate 162

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LQ158_RS06490 Protein ID WP_000911329.1
Coordinates 1335893..1336291 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LQ158_RS06485 Protein ID WP_000450524.1
Coordinates 1335666..1335893 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ158_RS06460 (1331468) 1331468..1331938 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
LQ158_RS06465 (1331938) 1331938..1332510 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LQ158_RS06470 (1332656) 1332656..1333534 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
LQ158_RS06475 (1333551) 1333551..1334585 + 1035 WP_023142816.1 outer membrane protein assembly factor BamC -
LQ158_RS06480 (1334798) 1334798..1335511 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LQ158_RS06485 (1335666) 1335666..1335893 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LQ158_RS06490 (1335893) 1335893..1336291 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ158_RS06495 (1336438) 1336438..1337301 + 864 WP_001267498.1 neutral zinc metallopeptidase -
LQ158_RS06500 (1337316) 1337316..1339331 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
LQ158_RS06505 (1339405) 1339405..1340103 + 699 WP_000679823.1 esterase -
LQ158_RS06510 (1340213) 1340213..1340413 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T295680 WP_000911329.1 NZ_OW849377:1335893-1336291 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T295680 NZ_OW849377:1335893-1336291 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT295680 WP_000450524.1 NZ_OW849377:1335666-1335893 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT295680 NZ_OW849377:1335666-1335893 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References