Detailed information of TA system TA029565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 43058..43605
• Replicon: plasmid pCAR1.3
• Accession: NC_021506
• Organism: Pseudomonas resinovorans NBRC 106553

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6AKK9
• Locus tag: PCA10_RS28380
• Protein ID: WP_016502317.1
• Coordinates: 43058..43333 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6AY38
• Locus tag: PCA10_RS28385
• Protein ID: WP_016502318.1
• Coordinates: 43333..43605 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCA10_RS29950 (PCA10_p0410)	38064..38453	-	390	WP_016502314.1	hypothetical protein	-
PCA10_RS28360 (PCA10_p0420)	38434..39231	-	798	WP_011077890.1	hypothetical protein	-
PCA10_RS28365 (PCA10_p0430)	39240..39650	-	411	WP_016502315.1	helix-turn-helix domain-containing protein	-
PCA10_RS29955 (PCA10_p0440)	39766..39972	+	207	WP_016502316.1	DNA-binding protein	-
PCA10_RS28370 (PCA10_p0450)	40266..41246	+	981	WP_011077862.1	IS5-like element ISPre1 family transposase	-
PCA10_RS28375 (PCA10_p0460)	41369..42394	+	1026	WP_144277083.1	bifunctional diguanylate cyclase/phosphodiesterase	-
PCA10_RS29960 (PCA10_p0470)	42645..42914	+	270	WP_011077892.1	hypothetical protein	-
PCA10_RS28380 (PCA10_p0480)	43058..43333	-	276	WP_016502317.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PCA10_RS28385 (PCA10_p0490)	43333..43605	-	273	WP_016502318.1	ribbon-helix-helix domain-containing protein	Antitoxin
PCA10_RS28390 (PCA10_p0500)	43619..44032	-	414	WP_016502319.1	hypothetical protein	-
PCA10_RS28395 (PCA10_p0510)	44039..44779	-	741	WP_011077894.1	DUF2807 domain-containing protein	-
PCA10_RS28400 (PCA10_p0520)	45011..45571	+	561	WP_011077895.1	recombinase family protein	-
PCA10_RS28405 (PCA10_p0530)	45575..46846	+	1272	Protein_52	DUF4158 domain-containing protein	-
PCA10_RS28410 (PCA10_p0540)	46933..47682	-	750	WP_013100941.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198965	198965
	-	inside	IScluster/Tn	-	-	40266..56437	16171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10702.22 Da
• Isoelectric Point: 7.2020

>T29565 WP_016502317.1 NC_021506:c43333-43058 [Pseudomonas resinovorans NBRC 106553]
MELKWTSKALSDIARLYEFLASVNQPAAARTVQQLTAAPSTLLANPRIGERLEEFDPRDVRRIQIGHYEMRYEIVDSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T29565 NC_021506:c43333-43058 [Pseudomonas resinovorans NBRC 106553]
ATGGAGTTGAAGTGGACAAGCAAGGCGCTTTCCGACATTGCCCGGTTGTATGAGTTTCTGGCGTCGGTGAATCAGCCTGC
CGCCGCACGAACAGTGCAGCAACTCACAGCCGCACCTTCCACGCTGCTGGCCAACCCGCGCATTGGCGAACGCCTGGAAG
AATTCGATCCACGGGATGTGCGCCGAATTCAGATCGGCCATTACGAGATGCGTTACGAGATAGTGGATTCCACTATCTAC
CTACTGCGCCTGTGGCACACCCGCGAAGACCGATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10074.43 Da
• Isoelectric Point: 5.7066

>AT29565 WP_016502318.1 NC_021506:c43605-43333 [Pseudomonas resinovorans NBRC 106553]
MATTAKTRSVTAHVPVQLAEKVDLMAERLERSKNWIVKQALAAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWAESL
STDTPLPVPR

Nucleotide

• Length: 273 bp

>AT29565 NC_021506:c43605-43333 [Pseudomonas resinovorans NBRC 106553]
ATGGCAACCACCGCGAAAACCCGATCCGTCACGGCTCACGTCCCTGTGCAACTGGCCGAAAAGGTCGATTTGATGGCTGA
GCGCCTGGAACGCTCCAAGAACTGGATCGTCAAGCAGGCTCTGGCCGCCTGGATAGACCAGGAAGAAGAACGTAGTCGCC
TGACCCGTGAGGCATTGGCCGATGTGGATGCTGGTCGCGTGATCGATCATCAAGCTGTCCAGGCCTGGGCTGAAAGCCTC
AGCACTGACACTCCGTTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: S6AKK9

Antitoxin

• AlphaFold DB: S6AY38