Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 107217..108020 Replicon plasmid P1
Accession NZ_OW849083
Organism Citrobacter freundii isolate 8

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag LQ154_RS26030 Protein ID WP_022652313.1
Coordinates 107217..107747 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag LQ154_RS26035 Protein ID WP_022652312.1
Coordinates 107751..108020 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ154_RS26005 (AI2642V1_REPA000000169) 103159..103635 + 477 Protein_118 IS3 family transposase -
LQ154_RS26010 (AI2642V1_REPA000000147) 103776..103934 - 159 Protein_119 IS5/IS1182 family transposase -
LQ154_RS26015 (AI2642V1_5077) 104027..105526 + 1500 WP_071444479.1 IS21-like element ISPlge2 family transposase -
LQ154_RS26020 (AI2642V1_5078) 105523..106287 + 765 WP_071444478.1 IS21-like element helper ATPase IstB -
LQ154_RS26025 (AI2642V1_5079) 106345..107049 - 705 Protein_122 IS5 family transposase -
LQ154_RS26030 (AI2642V1_5080) 107217..107747 - 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
LQ154_RS26035 (AI2642V1_5081) 107751..108020 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
LQ154_RS26040 (AI2642V1_5082) 108876..109865 + 990 WP_022652311.1 RepB family plasmid replication initiator protein -
LQ154_RS26045 (AI2642V1_5083) 110191..110931 - 741 WP_230129906.1 site-specific integrase -
LQ154_RS26050 (AI2642V1_REPA000000125) 111408..111715 + 308 Protein_127 IS1-like element transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..197744 197744
- inside IScluster/Tn - - 78669..113029 34360


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T295649 WP_022652313.1 NZ_OW849083:c107747-107217 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT295649 WP_022652312.1 NZ_OW849083:c108020-107751 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References