Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4949462..4950078 Replicon chromosome
Accession NZ_OW849082
Organism Citrobacter freundii isolate 8

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag LQ154_RS24475 Protein ID WP_003028682.1
Coordinates 4949462..4949836 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag LQ154_RS24480 Protein ID WP_043018956.1
Coordinates 4949836..4950078 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ154_RS24460 (4946965) 4946965..4947867 + 903 WP_106107909.1 formate dehydrogenase O subunit beta -
LQ154_RS24465 (4947864) 4947864..4948499 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
LQ154_RS24470 (4948496) 4948496..4949425 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
LQ154_RS24475 (4949462) 4949462..4949836 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ154_RS24480 (4949836) 4949836..4950078 - 243 WP_043018956.1 CopG family transcriptional regulator Antitoxin
LQ154_RS24485 (4950284) 4950284..4951192 + 909 WP_119174684.1 alpha/beta hydrolase -
LQ154_RS24490 (4951343) 4951343..4952284 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
LQ154_RS24495 (4952329) 4952329..4952766 - 438 WP_003028671.1 D-aminoacyl-tRNA deacylase -
LQ154_RS24500 (4952763) 4952763..4953635 - 873 WP_003840437.1 virulence factor BrkB family protein -
LQ154_RS24505 (4953629) 4953629..4954228 - 600 WP_016151258.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T295646 WP_003028682.1 NZ_OW849082:c4949836-4949462 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT295646 WP_043018956.1 NZ_OW849082:c4950078-4949836 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References