Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3055629..3056219 Replicon chromosome
Accession NZ_OW849082
Organism Citrobacter freundii isolate 8

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag LQ154_RS15235 Protein ID WP_003836692.1
Coordinates 3055629..3055961 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag LQ154_RS15240 Protein ID WP_003836694.1
Coordinates 3055962..3056219 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ154_RS15210 (3051382) 3051382..3052869 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
LQ154_RS15220 (3053185) 3053185..3054135 - 951 WP_119174146.1 HTH-type transcriptional regulator Cbl -
LQ154_RS15225 (3054237) 3054237..3055154 - 918 WP_044700649.1 nitrogen assimilation transcriptional regulator NAC -
LQ154_RS15235 (3055629) 3055629..3055961 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LQ154_RS15240 (3055962) 3055962..3056219 - 258 WP_003836694.1 hypothetical protein Antitoxin
LQ154_RS15245 (3056833) 3056833..3059829 + 2997 WP_230129733.1 hypothetical protein -
LQ154_RS15250 (3059913) 3059913..3060944 + 1032 WP_032425611.1 IS630-like element ISEc33 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3054237..3075159 20922


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T295642 WP_003836692.1 NZ_OW849082:c3055961-3055629 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT295642 WP_003836694.1 NZ_OW849082:c3056219-3055962 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References