Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 78629..79154 Replicon plasmid P1
Accession NZ_OW849065
Organism Escherichia coli isolate 131

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag LQ135_RS26040 Protein ID WP_001159868.1
Coordinates 78849..79154 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag LQ135_RS26035 Protein ID WP_000813634.1
Coordinates 78629..78847 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ135_RS26010 (73908) 73908..75521 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
LQ135_RS26015 (75552) 75552..75902 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
LQ135_RS26020 (75899) 75899..76324 - 426 WP_000422741.1 transposase -
LQ135_RS26025 (76416) 76416..77537 + 1122 WP_012783980.1 DUF3800 domain-containing protein -
LQ135_RS26030 (77571) 77571..78059 - 489 WP_011254646.1 hypothetical protein -
LQ135_RS26035 (78629) 78629..78847 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LQ135_RS26040 (78849) 78849..79154 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
LQ135_RS26045 (79155) 79155..79961 + 807 WP_000016982.1 site-specific integrase -
LQ135_RS26050 (80735) 80735..81490 + 756 WP_000852146.1 replication initiation protein RepE -
LQ135_RS26055 (82078) 82078..83244 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / aadA5 / qacE / sul1 - 1..146311 146311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T295628 WP_001159868.1 NZ_OW849065:78849-79154 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT295628 WP_000813634.1 NZ_OW849065:78629-78847 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References