295626

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 66640..67283 Replicon plasmid P1
Accession NZ_OW849065
Organism Escherichia coli isolate 131

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag LQ135_RS25975 Protein ID WP_001034044.1
Coordinates 66640..67056 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag LQ135_RS25980 Protein ID WP_001261286.1
Coordinates 67053..67283 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ135_RS25950 (62485) 62485..62753 + 269 Protein_77 type II toxin-antitoxin system ParD family antitoxin -
LQ135_RS25955 (62740) 62740..63008 + 269 Protein_78 type II toxin-antitoxin system RelE/ParE family toxin -
LQ135_RS25960 (63042) 63042..63739 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
LQ135_RS25965 (63993) 63993..65015 - 1023 WP_000361402.1 helicase UvrD -
LQ135_RS25970 (65000) 65000..66565 - 1566 WP_001128474.1 AAA family ATPase -
LQ135_RS25975 (66640) 66640..67056 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
LQ135_RS25980 (67053) 67053..67283 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LQ135_RS25985 (67664) 67664..71458 + 3795 WP_001144732.1 hypothetical protein -
LQ135_RS25990 (71503) 71503..71919 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
LQ135_RS25995 (71916) 71916..72146 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / aadA5 / qacE / sul1 - 1..146311 146311
- flank IS/Tn - - 63362..63739 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T295626 WP_001034044.1 NZ_OW849065:c67056-66640 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT295626 WP_001261286.1 NZ_OW849065:c67283-67053 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References