Detailed information of TA system TA295622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4512003..4513692
• Replicon: chromosome
• Accession: NZ_OW849064
• Organism: Escherichia coli isolate 131

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: LQ135_RS22270
• Protein ID: WP_000365807.1
• Coordinates: 4512772..4513692 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LQ135_RS22265
• Protein ID: WP_001295795.1
• Coordinates: 4512003..4512785 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LQ135_RS22260 (4508621)	4508621..4511719	-	3099	WP_000673070.1	type I restriction endonuclease	-
LQ135_RS22265 (4512003)	4512003..4512785	+	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LQ135_RS22270 (4512772)	4512772..4513692	+	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LQ135_RS22275 (4513857)	4513857..4514633	-	777	WP_000171155.1	site-specific integrase	-
LQ135_RS22280 (4514841)	4514841..4515176	-	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
LQ135_RS22285 (4515403)	4515403..4515561	-	159	Protein_4362	integrase	-
LQ135_RS22290 (4515610)	4515610..4515795	+	186	WP_000146240.1	hypothetical protein	-
LQ135_RS22295 (4516010)	4516010..4516624	+	615	WP_001019923.1	YagU family protein	-
LQ135_RS22300 (4516695)	4516695..4516861	+	167	Protein_4365	tail fiber assembly protein	-
LQ135_RS22305 (4516873)	4516873..4517202	-	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
LQ135_RS22310 (4517509)	4517509..4518219	-	711	WP_001295798.1	fimbrial chaperone EcpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	4505753..4553909	48156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T295622 WP_000365807.1 NZ_OW849064:4512772-4513692 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT295622 WP_001295795.1 NZ_OW849064:4512003-4512785 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

Structures

Toxin

• AlphaFold DB: A0A061KTE0