Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4135527..4136103 Replicon chromosome
Accession NZ_OW849064
Organism Escherichia coli isolate 131

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag LQ135_RS20450 Protein ID WP_001295743.1
Coordinates 4135527..4135814 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag LQ135_RS20455 Protein ID WP_000063148.1
Coordinates 4135801..4136103 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ135_RS20435 (4131187) 4131187..4132491 - 1305 WP_000535012.1 restriction endonuclease subunit S -
LQ135_RS20440 (4132481) 4132481..4134100 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
LQ135_RS20445 (4134164) 4134164..4135330 - 1167 WP_000800831.1 restriction endonuclease -
LQ135_RS20450 (4135527) 4135527..4135814 + 288 WP_001295743.1 BrnT family toxin Toxin
LQ135_RS20455 (4135801) 4135801..4136103 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
LQ135_RS20460 (4136137) 4136137..4137093 - 957 WP_001295745.1 GTPase -
LQ135_RS20465 (4137104) 4137104..4137307 - 204 WP_000467859.1 YbdD/YjiX family protein -
LQ135_RS20470 (4137357) 4137357..4139507 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T295619 WP_001295743.1 NZ_OW849064:4135527-4135814 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT295619 WP_000063148.1 NZ_OW849064:4135801-4136103 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References