Detailed information of TA system TA029555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 344885..345549
• Replicon: chromosome
• Accession: NC_021505
• Organism: Pseudomonas putida NBRC 14164

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PP4_RS27655
• Protein ID: WP_172488783.1
• Coordinates: 344885..345034 (+)
• Length: 50 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PP4_RS01505
• Protein ID: WP_016497583.1
• Coordinates: 345076..345549 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP4_RS01495	341076..341798	-	723	WP_016497580.1	response regulator	-
PP4_RS01500	341939..344236	+	2298	WP_016497581.1	TonB-dependent siderophore receptor	-
PP4_RS27650	344528..344734	-	207	WP_016497582.1	DUF3079 domain-containing protein	-
PP4_RS27655	344885..345034	+	150	WP_172488783.1	type II toxin-antitoxin system HigB family toxin	Toxin
PP4_RS01505	345076..345549	+	474	WP_016497583.1	transcriptional regulator	Antitoxin
PP4_RS01510	345959..347257	+	1299	WP_016497584.1	hypothetical protein	-
PP4_RS01515	347261..347839	+	579	WP_016497585.1	hypothetical protein	-
PP4_RS27660	348041..348814	-	774	WP_016497586.1	FAD-dependent thymidylate synthase	-
PP4_RS28475	348811..349701	-	891	WP_041167494.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (11-43)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5808.78 Da
• Isoelectric Point: 10.2378

>T29555 WP_172488783.1 NC_021505:344885-345034 [Pseudomonas putida NBRC 14164]
MLGRRDNTMRVKKNECRHIACLNARTGTLYIKAILSHAEYDKWCRSDIR

Nucleotide

• Length: 150 bp

>T29555 NC_021505:344885-345034 [Pseudomonas putida NBRC 14164]
ATGTTGGGGCGCCGCGACAACACAATGCGGGTCAAGAAAAACGAATGCCGCCACATCGCGTGTCTCAATGCGCGAACGGG
CACTCTGTACATCAAGGCCATTTTGTCGCACGCCGAGTACGACAAATGGTGCCGAAGCGATATCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17170.65 Da
• Isoelectric Point: 4.6719

>AT29555 WP_016497583.1 NC_021505:345076-345549 [Pseudomonas putida NBRC 14164]
MKRAKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELQARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQVKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT29555 NC_021505:345076-345549 [Pseudomonas putida NBRC 14164]
ATGAAGCGGGCTAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTGGCCGCCTGCCATGATCCCATCAAGGACGATGCCGAGCTTCAGGCACGCATGG
CATTGATCGACGAGCTTTACTCCCATGCGGACAGTGAAGGCCACGCTGCGGCACGCTTTGCGGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCCGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGACAG
GGGGGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTTTCGGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGGTAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTCGAGTTCTTCATGCATGGGGTGGTTTGA