Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 30519..31322 Replicon plasmid pKOX_NDM1
Accession NC_021501
Organism Klebsiella oxytoca E718

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag A225_RS30415 Protein ID WP_016479963.1
Coordinates 30792..31322 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag A225_RS30410 Protein ID WP_022652312.1
Coordinates 30519..30788 (+) Length 90 a.a.

Genomic Context

A225_RS30400A225_RS30410A225_RS30415A225_RS30420A225_RS33295A225_RS30405A225_RS32810A225_RS34025A225_RS34595A225_RS30440A225_RS30445
Location: 27639..28378 (740 bp)
Type: Others
Protein ID: Protein_26
Location: 30519..30788 (270 bp)
Type: Antitoxin
Protein ID: WP_022652312.1
Location: 30792..31322 (531 bp)
Type: Toxin
Protein ID: WP_016479963.1
Location: 31454..32470 (1017 bp)
Type: Others
Protein ID: WP_087728544.1
Location: 27005..27157 (153 bp)
Type: Others
Protein ID: Protein_25
Location: 28674..29663 (990 bp)
Type: Others
Protein ID: WP_022652311.1
Location: 32617..33366 (750 bp)
Type: Others
Protein ID: Protein_31
Location: 33376..33495 (120 bp)
Type: Others
Protein ID: Protein_32
Location: 33756..33932 (177 bp)
Type: Others
Protein ID: Protein_33
Location: 34121..35761 (1641 bp)
Type: Others
Protein ID: WP_004201176.1
Location: 35817..36107 (291 bp)
Type: Others
Protein ID: WP_004201172.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
A225_RS3329527005..27157-153Protein_25IS5/IS1182 family transposase-
A225_RS3040027639..28378+740Protein_26site-specific integrase-
A225_RS3040528674..29663-990WP_022652311.1RepB family plasmid replication initiator protein-
A225_RS3041030519..30788+270WP_022652312.1DUF1778 domain-containing proteinAntitoxin
A225_RS3041530792..31322+531WP_016479963.1GNAT family N-acetyltransferaseToxin
A225_RS3042031454..32470+1017WP_087728544.1IS5-like element IS5 family transposase-
A225_RS3281032617..33366-750Protein_31IS3 family transposase-
A225_RS3402533376..33495-120Protein_32IS30 family transposase-
A225_RS3459533756..33932-177Protein_33IS91 family transposase-
A225_RS3044034121..35761-1641WP_004201176.1chaperonin GroEL-
A225_RS3044535817..36107-291WP_004201172.1co-chaperone GroES-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideConjugative plasmidblaNDM-1 / rmtC / sul1htpB1..110781110781
-insideIScluster/Tn--5090..3328228192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T29554 WP_016479963.1 NC_021501:30792-31322 [Klebsiella michiganensis E718]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T29554 NC_021501:30792-31322 [Klebsiella michiganensis E718]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT29554 WP_022652312.1 NC_021501:30519-30788 [Klebsiella michiganensis E718]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT29554 NC_021501:30519-30788 [Klebsiella michiganensis E718]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found
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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
No matching records found

Structures

Toxin

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Source
ID
Structure
AlphaFold DBA0A6C0L7Q2

Antitoxin

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Source
ID
Structure
AlphaFold DBA0A2Z3XDS5

References