Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 30519..31322 Replicon plasmid pKOX_NDM1
Accession NC_021501
Organism Klebsiella oxytoca E718

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag A225_RS30415 Protein ID WP_016479963.1
Coordinates 30792..31322 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag A225_RS30410 Protein ID WP_022652312.1
Coordinates 30519..30788 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A225_RS33295 27005..27157 - 153 Protein_25 IS5/IS1182 family transposase -
A225_RS30400 27639..28378 + 740 Protein_26 site-specific integrase -
A225_RS30405 28674..29663 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
A225_RS30410 30519..30788 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
A225_RS30415 30792..31322 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
A225_RS30420 31454..32470 + 1017 WP_087728544.1 IS5-like element IS5 family transposase -
A225_RS32810 32617..33366 - 750 Protein_31 IS3 family transposase -
A225_RS34025 33376..33495 - 120 Protein_32 IS30 family transposase -
A225_RS34595 33756..33932 - 177 Protein_33 IS91 family transposase -
A225_RS30440 34121..35761 - 1641 WP_004201176.1 chaperonin GroEL -
A225_RS30445 35817..36107 - 291 WP_004201172.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / rmtC / sul1 htpB 1..110781 110781
- inside IScluster/Tn - - 5090..33282 28192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T29554 WP_016479963.1 NC_021501:30792-31322 [Klebsiella michiganensis E718]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T29554 NC_021501:30792-31322 [Klebsiella michiganensis E718]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT29554 WP_022652312.1 NC_021501:30519-30788 [Klebsiella michiganensis E718]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT29554 NC_021501:30519-30788 [Klebsiella michiganensis E718]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References