Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3524964..3525540 Replicon chromosome
Accession NZ_OW848785
Organism Escherichia coli isolate 131

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag LQ117_RS16975 Protein ID WP_001295743.1
Coordinates 3524964..3525251 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag LQ117_RS16980 Protein ID WP_000063148.1
Coordinates 3525238..3525540 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ117_RS16960 (3520624) 3520624..3521928 - 1305 WP_000535012.1 restriction endonuclease subunit S -
LQ117_RS16965 (3521918) 3521918..3523537 - 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
LQ117_RS16970 (3523601) 3523601..3524767 - 1167 WP_000800831.1 restriction endonuclease -
LQ117_RS16975 (3524964) 3524964..3525251 + 288 WP_001295743.1 BrnT family toxin Toxin
LQ117_RS16980 (3525238) 3525238..3525540 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
LQ117_RS16985 (3525574) 3525574..3526530 - 957 WP_001295745.1 GTPase -
LQ117_RS16990 (3526541) 3526541..3526744 - 204 WP_000467859.1 YbdD/YjiX family protein -
LQ117_RS16995 (3526794) 3526794..3528944 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T295532 WP_001295743.1 NZ_OW848785:3524964-3525251 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT295532 WP_000063148.1 NZ_OW848785:3525238-3525540 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References