Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21733..22258 Replicon plasmid pKOX_NDM1
Accession NC_021501
Organism Klebsiella oxytoca E718

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag A225_RS30360 Protein ID WP_008322213.1
Coordinates 21953..22258 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag A225_RS30355 Protein ID WP_032934863.1
Coordinates 21733..21951 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A225_RS30345 17379..19961 - 2583 WP_016479955.1 EAL domain-containing protein -
A225_RS32800 20174..20871 + 698 Protein_17 IS1 family transposase -
A225_RS30355 21733..21951 + 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A225_RS30360 21953..22258 + 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
A225_RS30365 22260..22574 + 315 WP_008322211.1 hypothetical protein -
A225_RS30370 22564..23355 + 792 WP_008322208.1 site-specific integrase -
A225_RS30375 23554..24876 - 1323 WP_016479957.1 GntP family transporter -
A225_RS30380 25038..25784 - 747 Protein_23 IS5 family transposase -
A225_RS33295 27005..27157 - 153 Protein_25 IS5/IS1182 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / rmtC / sul1 htpB 1..110781 110781
- inside IScluster/Tn - - 5090..33282 28192


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T29553 WP_008322213.1 NC_021501:21953-22258 [Klebsiella michiganensis E718]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T29553 NC_021501:21953-22258 [Klebsiella michiganensis E718]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT29553 WP_032934863.1 NC_021501:21733-21951 [Klebsiella michiganensis E718]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT29553 NC_021501:21733-21951 [Klebsiella michiganensis E718]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References