Detailed information of TA system TA029551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4429768..4430562
• Replicon: chromosome
• Accession: NC_021500
• Organism: Enterobacter sp. R4-368

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: H650_RS20630
• Protein ID: WP_020456981.1
• Coordinates: 4429768..4430289 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: H650_RS20635
• Protein ID: WP_020456982.1
• Coordinates: 4430293..4430562 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H650_RS20595	4424996..4425331	+	336	WP_020456974.1	SdpI family protein	-
H650_RS20600	4425331..4425603	+	273	WP_020456975.1	MafI family immunity protein	-
H650_RS20605	4425645..4425905	+	261	WP_020456976.1	hypothetical protein	-
H650_RS20610	4425979..4426431	-	453	WP_020456977.1	DUF1311 domain-containing protein	-
H650_RS20615	4426754..4428292	-	1539	WP_020456978.1	MCP four helix bundle domain-containing protein	-
H650_RS20620	4428729..4429301	-	573	WP_020456979.1	DUF1349 domain-containing protein	-
H650_RS20625	4429519..4429746	+	228	WP_020456980.1	helix-turn-helix domain-containing protein	-
H650_RS20630	4429768..4430289	-	522	WP_020456981.1	GNAT family N-acetyltransferase	Toxin
H650_RS20635	4430293..4430562	-	270	WP_020456982.1	DUF1778 domain-containing protein	Antitoxin
H650_RS20640	4430756..4431073	-	318	WP_020456983.1	hypothetical protein	-
H650_RS25795	4431316..4431489	+	174	WP_020456984.1	hypothetical protein	-
H650_RS20645	4431601..4433091	-	1491	WP_020456985.1	hypothetical protein	-
H650_RS20650	4433298..4433840	-	543	WP_020456986.1	acireductone dioxygenase	-
H650_RS20655	4433837..4434526	-	690	WP_020456987.1	acireductone synthase	-
H650_RS20660	4434523..4435137	-	615	WP_020456988.1	methylthioribulose 1-phosphate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19427.19 Da
• Isoelectric Point: 7.0496

>T29551 WP_020456981.1 NC_021500:c4430289-4429768 [Enterobacter sp. R4-368]
VDKLTIEMFSGETEYETGGFDCGEISLNTFLTQHLKRQHDGKILRAYILQTTASRPRVFGYYTLSGGSFEKEQLPTKTQQ
KKVPYRTVPCVMLGRLAIDKSLQGNGWGEMLVAHAMHVVYNASLAVGVHGLFVEALTDSAKNFYEKLGFIPLADGNQHSL
FYPTKSIERLFEE

Nucleotide

• Length: 522 bp

>T29551 NC_021500:c4430289-4429768 [Enterobacter sp. R4-368]
GTGGACAAGCTGACCATTGAGATGTTCTCAGGAGAGACAGAGTATGAAACGGGTGGTTTTGATTGTGGTGAGATCTCGCT
CAATACCTTTTTAACCCAGCATCTCAAGCGCCAGCATGACGGAAAAATTTTACGCGCCTATATCCTGCAAACAACAGCTT
CCAGGCCGCGCGTTTTTGGTTATTACACATTGTCGGGCGGGAGTTTCGAGAAAGAGCAACTGCCAACCAAAACGCAGCAA
AAAAAAGTGCCTTACCGCACTGTTCCGTGCGTGATGTTGGGTCGTCTGGCTATCGATAAATCACTCCAGGGAAACGGCTG
GGGAGAAATGCTGGTCGCACATGCCATGCACGTGGTTTACAACGCCTCACTTGCTGTCGGTGTCCATGGATTATTTGTCG
AGGCTCTCACGGACAGTGCCAAAAATTTTTATGAAAAACTGGGCTTCATTCCGTTAGCCGATGGTAATCAGCATTCGCTG
TTCTATCCGACAAAATCTATTGAGCGCCTGTTTGAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10240.73 Da
• Isoelectric Point: 10.0087

>AT29551 WP_020456982.1 NC_021500:c4430562-4430293 [Enterobacter sp. R4-368]
MPALKKQRIDLRLTDEDKKMIEEAAAMTNQTITQFLVSSASERAAEVIEQHRRLVLNEASWNRVMDAIENPPAPNDKLKR
AAKRLQNRE

Nucleotide

• Length: 270 bp

>AT29551 NC_021500:c4430562-4430293 [Enterobacter sp. R4-368]
ATGCCCGCACTGAAAAAGCAGCGCATCGATCTGCGATTGACCGACGAGGATAAAAAAATGATCGAAGAAGCGGCGGCAAT
GACCAACCAGACGATCACCCAATTTTTGGTGAGCAGCGCCTCGGAACGCGCCGCAGAAGTCATTGAACAGCATCGCCGTC
TGGTTCTGAATGAAGCGTCATGGAACCGGGTGATGGACGCTATCGAAAACCCACCCGCACCGAACGACAAGCTTAAACGC
GCCGCAAAACGTCTGCAAAACAGGGAGTGA