Detailed information of TA system TA029550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4227493..4228109
• Replicon: chromosome
• Accession: NC_021500
• Organism: Enterobacter sp. R4-368

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1G4ZCB1
• Locus tag: H650_RS19705
• Protein ID: WP_017459990.1
• Coordinates: 4227493..4227711 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: H650_RS19710
• Protein ID: WP_020456807.1
• Coordinates: 4227735..4228109 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H650_RS19675	4223653..4223913	+	261	WP_017459984.1	type B 50S ribosomal protein L31	-
H650_RS19680	4223917..4224057	+	141	WP_025263710.1	type B 50S ribosomal protein L36	-
H650_RS19685	4224054..4224755	-	702	WP_020456803.1	GNAT family N-acetyltransferase	-
H650_RS19690	4224856..4226304	+	1449	WP_020456804.1	PLP-dependent aminotransferase family protein	-
H650_RS19695	4226270..4226749	-	480	WP_020456805.1	YlaC family protein	-
H650_RS19700	4226850..4227416	-	567	WP_020456806.1	maltose O-acetyltransferase	-
H650_RS19705	4227493..4227711	-	219	WP_017459990.1	hemolysin expression modulator Hha	Toxin
H650_RS19710	4227735..4228109	-	375	WP_020456807.1	Hha toxicity modulator TomB	Antitoxin
H650_RS19715	4228637..4231786	-	3150	WP_020456808.1	multidrug efflux RND transporter permease subunit	-
H650_RS19720	4231809..4233002	-	1194	WP_020456809.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8651.08 Da
• Isoelectric Point: 7.9893

>T29550 WP_017459990.1 NC_021500:c4227711-4227493 [Enterobacter sp. R4-368]
MSEKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Nucleotide

• Length: 219 bp

>T29550 NC_021500:c4227711-4227493 [Enterobacter sp. R4-368]
ATGTCCGAAAAACCATTAACAAAAATTGATTATTTGATGCGTTTAAGACGCTGTCAGACAATTGATACCCTTGAACGTGT
AATTGAAAAAAATAAATATGAACTTCCCGACGAGGAACTCGCGGTGTTTTATTCAGCAGCGGATCATCGTCTGGCAGAAC
TGACCATGAATAAGCTTTATGACAAAATCCCCTCCTCCGTGTGGAAGTTTGTGCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14550.41 Da
• Isoelectric Point: 5.8346

>AT29550 WP_020456807.1 NC_021500:c4228109-4227735 [Enterobacter sp. R4-368]
MDEYSPKRHDIAQLKFLCETLYHDCIVNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIELIDEYL
DDTFMLFSSYGINTHDLQKWRKTGNKLFRSFVNVSKANPVSHSC

Nucleotide

• Length: 375 bp

>AT29550 NC_021500:c4228109-4227735 [Enterobacter sp. R4-368]
ATGGACGAATACTCGCCGAAAAGGCATGATATTGCGCAGCTCAAATTTCTCTGCGAAACGTTGTACCATGATTGCATCGT
TAATCTTGAAGAGAGCCATCATGGATGGGTTAACGATCCTACTTCAGCAATCAATTTGCAGTTAAACGAGCTGATAGAAC
ACATAGCCACTTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAACTGATAGACGAATACCTC
GACGATACGTTTATGTTGTTCAGTAGCTATGGCATAAATACACACGACTTACAGAAATGGCGTAAAACAGGTAACAAATT
GTTCCGCAGTTTCGTCAATGTAAGCAAGGCTAATCCTGTTAGCCACTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1G4ZCB1