Detailed information of TA system TA295306

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 3534705..3536479
• Replicon: chromosome
• Accession: NZ_OW052570
• Organism: Mycobacterium tuberculosis strain Lineage 1.1.2 isolate Mycobacterium tuberculosis

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: MR177_RS16275
• Protein ID: WP_003414409.1
• Coordinates: 3535595..3536479 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: MR177_RS16270
• Protein ID: WP_003414414.1
• Coordinates: 3534705..3535592 (+)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MR177_RS16240	3531007..3532089	+	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
MR177_RS16245	3532168..3532917	-	750	WP_003414497.1	enoyl-CoA hydratase	-
MR177_RS16250	3532964..3533179	+	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MR177_RS16255	3533176..3533568	+	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
MR177_RS16260	3533589..3533858	+	270	WP_003912856.1	DUF2277 family protein	-
MR177_RS16265	3533855..3534400	+	546	WP_242302794.1	DUF1802 family protein	-
MR177_RS16270	3534705..3535592	+	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
MR177_RS16275	3535595..3536479	+	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MR177_RS16280	3536751..3537296	+	546	WP_003904931.1	DUF1802 family protein	-
MR177_RS16285	3537630..3538418	+	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MR177_RS16290	3538415..3540853	+	2439	WP_003900580.1	type III-A CRISPR-associated protein Cas10/Csm1	-
MR177_RS16295	3540850..3541224	+	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T295306 WP_003414409.1 NZ_OW052570:3535595-3536479 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT295306 WP_003414414.1 NZ_OW052570:3534705-3535592 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9