Detailed information of TA system TA029530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 5235006..5235594
• Replicon: chromosome
• Accession: NC_021491
• Organism: Pseudomonas putida H8234

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: R9VGK1
• Locus tag: L483_RS24245
• Protein ID: WP_016488542.1
• Coordinates: 5235006..5235308 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: L483_RS24250
• Protein ID: WP_016488543.1
• Coordinates: 5235301..5235594 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L483_RS24225	5231185..5232078	-	894	WP_016488538.1	LysR family transcriptional regulator	-
L483_RS24230	5232185..5233354	+	1170	WP_016488539.1	MFS transporter	-
L483_RS24235	5233402..5234328	-	927	WP_016488540.1	DMT family transporter	-
L483_RS24240	5234435..5234830	-	396	WP_016488541.1	transcriptional regulator	-
L483_RS24245	5235006..5235308	+	303	WP_016488542.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L483_RS24250	5235301..5235594	+	294	WP_016488543.1	putative addiction module antidote protein	Antitoxin
L483_RS24255	5235684..5235956	+	273	WP_016488544.1	hypothetical protein	-
L483_RS24260	5236078..5237439	-	1362	WP_016488545.1	DEAD/DEAH box helicase	-
L483_RS24265	5237543..5238844	+	1302	WP_016488546.1	mechanosensitive ion channel family protein	-
L483_RS24270	5238953..5239888	-	936	WP_016488547.1	AEC family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11379.12 Da
• Isoelectric Point: 10.8638

>T29530 WP_016488542.1 NC_021491:5235006-5235308 [Pseudomonas putida H8234]
MKKIESSSFRHWVTGLRDLSARARIISRINRLMEGLPGDVSPVGHGVSELKIHYGPGYRVYFHQTGSTFVILLCGGDKSS
QKRDIKVAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T29530 NC_021491:5235006-5235308 [Pseudomonas putida H8234]
ATGAAGAAAATCGAATCCAGCAGTTTCAGGCATTGGGTGACCGGACTACGGGACTTAAGCGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGTCTGCCGGGCGACGTATCTCCGGTAGGCCATGGCGTGAGTGAGCTCAAAATTCACT
ATGGTCCCGGCTATCGGGTGTACTTCCATCAGACGGGCAGCACCTTCGTCATTCTGCTCTGTGGCGGCGACAAAAGCAGT
CAAAAGCGAGACATCAAGGTTGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10446.88 Da
• Isoelectric Point: 5.0637

>AT29530 WP_016488543.1 NC_021491:5235301-5235594 [Pseudomonas putida H8234]
MTETFSEFDPAAYLTTPEAIAQFMTDALETGDASYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTARPHLAG

Nucleotide

• Length: 294 bp

>AT29530 NC_021491:5235301-5235594 [Pseudomonas putida H8234]
ATGACTGAAACCTTCTCTGAATTCGATCCTGCCGCGTACCTCACTACACCTGAAGCAATCGCGCAGTTCATGACCGATGC
ACTTGAAACCGGCGATGCCAGCTATGTCGCTAAAGCTGTCGGCGTGGTTGCACGTGCCAAGGGCATGAGCGAACTGGCGA
AAGAGACGGGGCTGTCCCGAGAGCAGCTTTACAGGTCATTCAGCGAGCATGGCAACCCCACGCTGAAGTCGTTTCTGACC
GTGATGAAGGCATTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Structures

Toxin

• AlphaFold DB: R9VGK1