Detailed information of TA system TA029528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 4229681..4230564
• Replicon: chromosome
• Accession: NC_021491
• Organism: Pseudomonas putida H8234

Toxin (Protein)

• Gene name: -
• Uniprot ID: R9VG37
• Locus tag: L483_RS19570
• Protein ID: WP_016487683.1
• Coordinates: 4229681..4230118 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R9VFG2
• Locus tag: L483_RS19575
• Protein ID: WP_016487684.1
• Coordinates: 4230115..4230564 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L483_RS33460	4224876..4225259	-	384	WP_095115706.1	hypothetical protein	-
L483_RS19550	4225261..4225947	-	687	Protein_3904	hypothetical protein	-
L483_RS19555	4226030..4227259	-	1230	WP_016487680.1	acyl-CoA dehydrogenase	-
L483_RS19560	4227399..4228325	+	927	WP_016487681.1	LysR family transcriptional regulator	-
L483_RS19565	4228519..4229673	+	1155	WP_016487682.1	aminotransferase class V-fold PLP-dependent enzyme	-
L483_RS19570	4229681..4230118	-	438	WP_016487683.1	RES family NAD+ phosphorylase	Toxin
L483_RS19575	4230115..4230564	-	450	WP_016487684.1	MbcA/ParS/Xre antitoxin family protein	Antitoxin
L483_RS19580	4230671..4231447	-	777	WP_016487685.1	SDR family oxidoreductase	-
L483_RS19585	4231591..4232511	-	921	WP_016487686.1	LysR family transcriptional regulator	-
L483_RS19590	4232804..4233526	-	723	WP_016487687.1	hypothetical protein	-
L483_RS19595	4233606..4233905	-	300	WP_016487688.1	DUF3077 domain-containing protein	-
L483_RS19600	4234105..4234728	+	624	WP_016487689.1	glutathione S-transferase	-
L483_RS33975	4234835..4234972	+	138	WP_157754405.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 16005.35 Da
• Isoelectric Point: 6.2187

>T29528 WP_016487683.1 NC_021491:c4230118-4229681 [Pseudomonas putida H8234]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPSGAMLEVLVHLEIDPEDFPTTMRLIRIELPDTVSQAQLPALQ
PGWSAQPELTRALGNRFLDDCSALLLPVPSAIMPSTINYLFNPRHAQAQRAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T29528 NC_021491:c4230118-4229681 [Pseudomonas putida H8234]
GTGATTTTGTGGCGTATCAGCGCCTATGCAGATTTGAGCGGCACTGGGGGGCTGCGTGTTAGCGGACGCTGGCACCAGGC
GGGCCGGCCCGTGGTGTATGCCGCCACCAGCCCTTCCGGGGCGATGCTCGAGGTGCTTGTGCACCTGGAGATCGACCCAG
AGGATTTTCCTACCACCATGCGCCTGATCCGTATCGAATTGCCTGATACGGTTTCACAGGCGCAGTTGCCCGCCTTGCAG
CCCGGCTGGTCCGCTCAGCCTGAGTTGACCCGGGCGCTGGGCAATCGCTTTCTGGATGACTGCTCGGCTTTACTGCTGCC
CGTGCCGAGCGCGATCATGCCCAGCACCATCAACTACCTGTTCAACCCACGGCACGCGCAGGCACAGCGTGCGAAAATCC
AGGTCGAGGATTTTACCCCGGACAGCCGCTTGTTCTAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17096.64 Da
• Isoelectric Point: 6.1089

>AT29528 WP_016487684.1 NC_021491:c4230564-4230115 [Pseudomonas putida H8234]
MLAEVLRDNGYHEYRARLQALLEIPELASDFEIHTRITHGFAATWLVKLTERGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEADKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT29528 NC_021491:c4230564-4230115 [Pseudomonas putida H8234]
ATGCTTGCCGAAGTGCTTCGTGACAACGGTTACCACGAGTACCGGGCGCGCCTGCAGGCGCTGCTGGAAATACCCGAACT
TGCCAGTGACTTCGAAATCCACACCCGGATCACCCACGGTTTTGCCGCTACCTGGCTGGTGAAGCTGACCGAGCGCGGTG
TGTTCACGCCGGTGGAGCGGGACCAGATCATTCCCCTGCGCACCCTGAAAACCCGTATCGAGCGTGACCAGCCGCTGACC
GTGGAAGAGAGTGATCGGCTGTTCCGTTCGGCCCACATCACCGCCATGGCCGAAGCCGTGTTTGGCGAGGCGGACAAGGC
CAAGCGCTGGCTGTCCAAGCCCAAGGAACGTTTTTCGGGGCTGACGCCGATGCAGATGCTCACAACCCAGCAAGGCACCA
CTCAGGTCGAAGAGATGCTGCTGCAGATTGCCGAGGGTTTTGGCCTGTGA

Structures

Toxin

• AlphaFold DB: R9VG37

Antitoxin

• AlphaFold DB: R9VFG2