Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3640595..3641165 Replicon chromosome
Accession NZ_OW052302
Organism Mycobacterium tuberculosis strain Lineage 3.1.1 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag MR176_RS16745 Protein ID WP_003414166.1
Coordinates 3640809..3641165 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag MR176_RS16740 Protein ID WP_003901465.1
Coordinates 3640595..3640825 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MR176_RS16700 3636072..3636299 - 228 Protein_3304 hypothetical protein -
MR176_RS16705 3636533..3636706 - 174 WP_234713711.1 hypothetical protein -
MR176_RS16710 3637408..3637668 - 261 Protein_3306 transposase -
MR176_RS16715 3637885..3638076 - 192 WP_003414184.1 hypothetical protein -
MR176_RS16720 3638073..3638465 - 393 WP_078397589.1 hypothetical protein -
MR176_RS16725 3638613..3638867 + 255 WP_155936531.1 hypothetical protein -
MR176_RS16730 3639043..3639510 - 468 WP_003414177.1 DUF1778 domain-containing protein -
MR176_RS16735 3639509..3640552 + 1044 WP_016720604.1 DUF2293 domain-containing protein -
MR176_RS16740 3640595..3640825 + 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
MR176_RS16745 3640809..3641165 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
MR176_RS16750 3641267..3642916 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
MR176_RS16755 3642935..3643522 - 588 WP_003914429.1 DUF3558 family protein -
MR176_RS16760 3643695..3644021 + 327 WP_003414157.1 hypothetical protein -
MR176_RS16765 3644025..3645713 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3613990..3647516 33526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T295248 WP_003414166.1 NZ_OW052302:3640809-3641165 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT295248 WP_003901465.1 NZ_OW052302:3640595-3640825 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References