Detailed information of TA system TA029522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 17098..17733
• Replicon: plasmid pA506
• Accession: NC_021361
• Organism: Pseudomonas fluorescens A506

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PFLA506_RS27025
• Protein ID: WP_016359316.1
• Coordinates: 17098..17280 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PFLA506_RS27030
• Protein ID: WP_016359324.1
• Coordinates: 17311..17733 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFLA506_RS26995	12261..12602	-	342	WP_016359361.1	hypothetical protein	-
PFLA506_RS27000	12599..13306	-	708	WP_016359334.1	ParA family protein	-
PFLA506_RS27005	14314..14733	+	420	WP_016359360.1	hypothetical protein	-
PFLA506_RS27015	15010..15327	+	318	WP_016359358.1	hypothetical protein	-
PFLA506_RS27020	15472..17019	+	1548	WP_144269802.1	KlcB family protein	-
PFLA506_RS27025	17098..17280	+	183	WP_016359316.1	type II toxin-antitoxin system HicA family toxin	Toxin
PFLA506_RS27030	17311..17733	+	423	WP_016359324.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PFLA506_RS27035	17776..18009	+	234	WP_016359340.1	hypothetical protein	-
PFLA506_RS27040	18049..18354	+	306	WP_016359337.1	hypothetical protein	-
PFLA506_RS27045	18351..18629	+	279	WP_016359331.1	hypothetical protein	-
PFLA506_RS27050	18645..18875	+	231	WP_016359322.1	hypothetical protein	-
PFLA506_RS27055	19010..19483	-	474	WP_044483786.1	hypothetical protein	-
PFLA506_RS27060	19614..19952	-	339	WP_016359307.1	helix-turn-helix transcriptional regulator	-
PFLA506_RS27065	20215..20730	+	516	WP_016359370.1	transcription termination factor, NusG family	-
PFLA506_RS27070	20816..21028	+	213	WP_044483787.1	hypothetical protein	-
PFLA506_RS27075	21530..21853	+	324	WP_016359336.1	hypothetical protein	-
PFLA506_RS28130	21883..22305	+	423	WP_016359339.1	VirB3 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56977	56977

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6882.04 Da
• Isoelectric Point: 10.5498

>T29522 WP_016359316.1 NC_021361:17098-17280 [Pseudomonas fluorescens A506]
MKCSEFRRWLLAQGVEFKSAKGSHFKVYLNGKSTIFADHGSKEMHEGLRKTIIKQLGLKD

Nucleotide

• Length: 183 bp

>T29522 NC_021361:17098-17280 [Pseudomonas fluorescens A506]
ATGAAGTGCAGCGAGTTTCGGCGTTGGTTGTTGGCCCAAGGCGTAGAGTTCAAAAGCGCCAAGGGCAGTCACTTCAAAGT
TTATTTGAATGGCAAATCGACCATCTTTGCGGACCATGGCAGCAAGGAAATGCACGAAGGGTTACGCAAAACCATTATCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15523.96 Da
• Isoelectric Point: 5.2401

>AT29522 WP_016359324.1 NC_021361:17311-17733 [Pseudomonas fluorescens A506]
MYQYPLELHHEATGVWLSCPDIPEMNASGDTLAEAFAEALDGFESALSLYVEQRRAIPRASAPTDPALVLRLPALTVAKI
MLWNAMVDEGVNRAELARRMGIKRQVAERLVDFLHASKIEQVERALSLLGRRISLVLEAA

Nucleotide

• Length: 423 bp

>AT29522 NC_021361:17311-17733 [Pseudomonas fluorescens A506]
ATGTATCAGTACCCGCTGGAGTTGCACCACGAAGCCACCGGCGTGTGGCTGTCGTGCCCTGATATACCTGAAATGAACGC
GTCCGGCGATACCCTGGCCGAAGCCTTTGCCGAGGCTCTGGACGGTTTTGAGTCGGCCCTGTCGCTGTACGTTGAGCAGC
GCCGCGCGATCCCGCGCGCCTCGGCTCCCACCGACCCGGCGTTGGTGCTGCGCTTGCCAGCGCTGACCGTGGCAAAAATC
ATGCTGTGGAACGCCATGGTGGACGAAGGCGTCAACCGCGCCGAGCTGGCCCGCCGCATGGGTATCAAACGGCAAGTCGC
TGAGAGGCTGGTGGACTTCCTGCACGCCTCGAAAATCGAGCAGGTAGAGCGCGCACTGAGCCTGTTGGGCCGGCGTATAT
CCCTAGTGCTGGAAGCGGCCTAG