Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 83943..84529 Replicon chromosome
Accession NZ_OW052302
Organism Mycobacterium tuberculosis strain Lineage 3.1.1 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag MR176_RS00350 Protein ID WP_003410010.1
Coordinates 84185..84529 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag MR176_RS00345 Protein ID WP_003410014.1
Coordinates 83943..84191 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MR176_RS00325 79989..80756 - 768 WP_003410019.1 hemerythrin domain-containing protein -
MR176_RS00330 80913..81269 + 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
MR176_RS00335 81322..81594 + 273 WP_003410017.1 DUF1490 family protein -
MR176_RS00340 81591..83843 + 2253 WP_016719189.1 cation transporter ATPase CptG -
MR176_RS00345 83943..84191 + 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
MR176_RS00350 84185..84529 + 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
MR176_RS00355 84618..84953 + 336 WP_003410009.1 dehydrogenase -
MR176_RS00360 84854..85111 - 258 WP_003410006.1 hypothetical protein -
MR176_RS00365 85197..85538 + 342 WP_003410003.1 DUF2384 domain-containing protein -
MR176_RS00370 85535..86095 + 561 WP_003410001.1 RES family NAD+ phosphorylase -
MR176_RS00375 86615..87154 - 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
MR176_RS00380 87380..87808 - 429 WP_003409992.1 cellulose-binding protein -
MR176_RS00385 88224..88823 - 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T295203 WP_003410010.1 NZ_OW052302:84185-84529 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT295203 WP_003410014.1 NZ_OW052302:83943-84191 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References