Detailed information of TA system TA029519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-YefM
• Location: 989850..990444
• Replicon: chromosome
• Accession: NC_021291
• Organism: Spiribacter salinus M19-40

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4V7T7
• Locus tag: SPISAL_RS04935
• Protein ID: WP_016353372.1
• Coordinates: 990184..990444 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4V549
• Locus tag: SPISAL_RS04930
• Protein ID: WP_016353371.1
• Coordinates: 989850..990101 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPISAL_RS08895	985067..985216	-	150	WP_016353364.1	hypothetical protein	-
SPISAL_RS04900	985379..985780	+	402	WP_016353365.1	hypothetical protein	-
SPISAL_RS04905	985828..986400	+	573	WP_016353366.1	hypothetical protein	-
SPISAL_RS04910	986637..987279	+	643	Protein_960	class I SAM-dependent methyltransferase	-
SPISAL_RS04920	987888..989585	+	1698	WP_016353369.1	gamma-glutamyltransferase	-
SPISAL_RS04925	989599..989853	-	255	WP_016353370.1	Txe/YoeB family addiction module toxin	-
SPISAL_RS04930	989850..990101	-	252	WP_016353371.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SPISAL_RS04935	990184..990444	-	261	WP_016353372.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SPISAL_RS04940	990431..990664	-	234	WP_016353373.1	hypothetical protein	-
SPISAL_RS04945	990740..991702	-	963	WP_016353374.1	alpha/beta hydrolase	-
SPISAL_RS04950	991885..992424	+	540	WP_016353375.1	porin family protein	-
SPISAL_RS04955	992536..993087	+	552	WP_016353376.1	histidine phosphatase family protein	-
SPISAL_RS04960	993072..993536	+	465	WP_051111890.1	isoprenylcysteine carboxylmethyltransferase family protein	-
SPISAL_RS04965	993527..994495	+	969	WP_016353378.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9934.56 Da
• Isoelectric Point: 10.6908

>T29519 WP_016353372.1 NC_021291:c990444-990184 [Spiribacter salinus M19-40]
MEKYELQFKRSVAKDLRGIPKPDVARILERISALSNEPRPPGSEKLTGEDRYRIRQGVYRIVYEIADAALIVTVVKAGHR
RQVCRR

Nucleotide

• Length: 261 bp

>T29519 NC_021291:c990444-990184 [Spiribacter salinus M19-40]
ATGGAAAAATATGAGCTGCAGTTCAAGCGCTCAGTCGCAAAGGATCTGCGGGGAATTCCCAAGCCCGACGTTGCAAGAAT
CCTTGAGCGCATTAGCGCGCTAAGCAATGAGCCCCGACCACCAGGGAGTGAGAAGCTCACTGGCGAGGATCGGTATCGGA
TCCGGCAGGGCGTCTATCGGATCGTTTATGAAATTGCTGACGCTGCTTTGATTGTGACAGTCGTCAAAGCCGGGCATCGG
AGGCAGGTCTGCCGACGCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9156.40 Da
• Isoelectric Point: 4.3219

>AT29519 WP_016353371.1 NC_021291:c990101-989850 [Spiribacter salinus M19-40]
MDAISYTAARSNLAKTMEQVCEDHSPVIITRSKAESVVMMSLADYEALQETAYLLRAPKNAQRLLEAVAELEQGDGQEKA
LIE

Nucleotide

• Length: 252 bp

>AT29519 NC_021291:c990101-989850 [Spiribacter salinus M19-40]
ATGGACGCCATCAGCTACACGGCGGCTCGATCGAATCTCGCAAAGACCATGGAGCAGGTGTGTGAAGACCACTCGCCGGT
GATCATTACCCGCAGCAAGGCCGAATCCGTGGTCATGATGTCTCTGGCTGACTACGAAGCGCTACAAGAAACGGCCTACC
TCCTGCGGGCGCCGAAGAACGCTCAGCGTTTGCTGGAAGCCGTTGCCGAGCTGGAGCAGGGCGATGGGCAAGAGAAGGCG
CTGATTGAATGA

Structures

Toxin

• AlphaFold DB: R4V7T7

Antitoxin

• AlphaFold DB: R4V549