Detailed information of TA system TA295114

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 2757430..2759204
• Replicon: chromosome
• Accession: NZ_OW052188
• Organism: Mycobacterium tuberculosis strain Lineage 1.1.2 isolate Mycobacterium tuberculosis

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: P71626
• Locus tag: MR221_RS12710
• Protein ID: WP_003414409.1
• Coordinates: 2758320..2759204 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: P71625
• Locus tag: MR221_RS12705
• Protein ID: WP_003414414.1
• Coordinates: 2757430..2758317 (+)
• Length: 296 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MR221_RS12675	2753732..2754814	+	1083	WP_003414499.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
MR221_RS12680	2754893..2755642	-	750	WP_003414497.1	enoyl-CoA hydratase	-
MR221_RS12685	2755689..2755904	+	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
MR221_RS12690	2755901..2756293	+	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	-
MR221_RS12695	2756314..2756583	+	270	WP_003414489.1	DUF2277 family protein	-
MR221_RS12700	2756580..2757125	+	546	WP_003908074.1	DUF1802 family protein	-
MR221_RS12705	2757430..2758317	+	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
MR221_RS12710	2758320..2759204	+	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MR221_RS12715	2759476..2760021	+	546	WP_003904931.1	DUF1802 family protein	-
MR221_RS12720	2760355..2761143	+	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
MR221_RS12725	2761140..2763578	+	2439	WP_003900580.1	type III-A CRISPR-associated protein Cas10/Csm1	-
MR221_RS12730	2763575..2763949	+	375	WP_003414296.1	type III-A CRISPR-associated protein Csm2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-272)

Antitoxin

• AbiEi antitoxin C-terminal domain (84-237)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33056.72 Da
• Isoelectric Point: 7.1520

>T295114 WP_003414409.1 NZ_OW052188:2758320-2759204 [Mycobacterium tuberculosis]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Nucleotide

• Length: 885 bp

Antitoxin

Protein

• Length: 296 a.a.
• Molecular weight: 32315.19 Da
• Isoelectric Point: 9.4723

>AT295114 WP_003414414.1 NZ_OW052188:2757430-2758317 [Mycobacterium tuberculosis]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Nucleotide

• Length: 888 bp

Structures

Toxin

• PDB: 6Y56

Antitoxin

• AlphaFold DB: A0A829CBU9